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Amino acid analysis by high-pressure liquid chromatography (HPLC)

For the absolute quantification of amino acids we use our Agilent 1290 HPLC system. The derivatised amino acids are separated by a C18 column in a nine minute gradient and detected by the Infinity Diode Array Detector (DAD) from Agilent. This system allows precise absolute quantification over a huge dynamic range.