Metabolite analysis by gas-chromatography coupled to time-of-flight mass spectrometry (GC-MS)

 

Our current GC-MS device is a 7200 accurate mass Q-TOF GC/MS from Agilent Technologies. We routinely quantify 50 metabolites, mainly of the central carbon metabolism in various species ranging from photosynthetic organism such as ArabidopsisFlaveriaCleomeZea mays, different water plants, red algae and cyanobacteria to yeast, Elysia chlorotica and serum from Canis lupus. The metabolite pattern found in different species or tissues, under different environmental conditions or genetic backgrounds in combination with physiological, transcriptional and/or enzymatic analyses plays a key role in understanding the species’ metabolism under the applied conditions.

 

Elemental analysis by gas source stable isotope ratio mass spectrometery (EA-IRMS)

With our IsoPrime100 from Isoprime we can analyse C and N content and C to N ratio and carbon isotope ratio (δ13C). δ13C of plant material, for example, is used to distinguish different pathways of carbon fixation such as C3, C3-C4 and C4 photosynthesis.

Amino acid analysis by high-pressure liquid chromatography (HPLC)

For the absolute quantification of amino acids we use our Agilent 1290 HPLC system. The derivatised amino acids are separated by a C18 column in a nine minute gradient and detected by the Infinity Diode Array Detector (DAD) from Agilent. This system allows precise absolute quantification over a huge dynamic range.

 

Team

Dr. Tabea Mettler-Altmann

Katrin Weber

Elisabeth Klemp

Maria Graf

Technical assistant

Katrin Weber

Technical assistant

Elisabeth Klemp

Technical assistant

Maria Graf

Responsible for the content: E-MailProf.Dr. Andreas Paul M. Weber